Coding
DnaJ/GrpE

Part:BBa_K205003:Design

Designed by: Richard Schlegel   Group: iGEM09_UQ-Australia   (2009-09-28)

DnaJ and GrpE are chaperone proteins that assists with polypeptide folding


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 609
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 111
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1605
    Illegal AgeI site found at 1697
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1378
    Illegal SapI.rc site found at 759


Design Notes

Gene was provided by Christos Kyratsous.

Source

Christos Kyratsous Columbia Univ, Coll Phys & Surg, Dept Microbiol, New York, NY 10032 USA

Ref: Kyratsous, CA, Silverstein, SJ, DeLong, CR, Panagiotidis, CA. (2009) "Chaperone-fusion expression plasmid vectors for improved solubility of recombinant proteins in Escherichia coli" GENE VOL: 440 Issue: 1-2 Pages: 9-15 Date: Jul 1 2009

References

DnaJ: http://www.uniprot.org/uniprot/P08622

GrpE: http://www.uniprot.org/uniprot/P09372